前警卫艾莉丝和环保人士马特·安德森在上一部电影中经过一系列战斗设法逃出丧尸爆发源头，地下遗传研究设施“蜂巢”。两人打算揭露安布雷拉制药公司在蜂巢的非法实验，但电影最后都落入安布雷拉控制。
安布雷拉派工作组前往蜂巢调查情况，但丧尸很快脱离控制，并因工作组打开封锁向附近的浣熊市蔓延。安布雷拉立刻将公司重要人员转移并隔离城市，研究员查尔斯·阿什福德博士之女儿安吉拉·阿什福德转移期间遇车祸失踪。与此同时，浣熊市警察局特别战术和救援组特工吉尔·瓦伦汀在停职期间返回前分局，向同僚建议撤出浣熊市。艾莉丝在废弃医院苏醒，然后在市里四处寻找物资，安布雷拉同时用唯一通往外界的桥梁疏散平民。瓦伦汀在桥上遇到前搭档佩顿·韦尔斯警佐，但他被丧尸咬伤并感染。由于病毒已蔓延到桥上，安布雷拉驻浣熊市部队指挥蒂莫西·凯恩少校下令封锁出口，强迫居民返回市内。
安布雷拉部队军人卡洛斯·奥利维拉和尼古拉·齐诺比耶夫尝试营救平民，但白忙一场之余还被部队抛弃，两人与幸存的特别战术和救援组特工反击丧尸又很快就被击溃，奥利维拉也被咬伤感染。瓦伦汀、韦尔斯和新闻主播特里·莫拉莱斯躲藏时碰到丧尸，幸得艾莉丝所救。安布雷拉派出严重变异的超级士兵实验体追迹者杀死残留的特别战术和救援组特工并追杀艾莉丝。阿什福德博士侵入城市闭路电视系统并联络艾莉丝及其他幸存者，声称只要帮忙找到安吉拉，自己会安排大家撤出浣熊市。他还向奥利维拉和齐诺比耶夫提出同等条件，称安布雷拉打算动用核弹摧毁浣熊市，一劳永逸地摆脱丧尸问题。
艾莉丝等人前往安吉拉所在地，但在路上遇到追迹者。瓦伦汀杀掉变成丧尸的韦尔斯，艾莉丝被追迹者打伤，只能分头逃跑，确保其他人脱险。瓦伦汀和莫拉莱斯继续前行，路上救出被困平民L.J.。瓦伦汀碰到奥利维拉，众人救出安吉拉，但莫拉莱斯和齐诺比耶夫被杀。安吉拉承认丧尸爆发是因父亲创造的病毒导致，但博士此举是为治疗女儿的遗传病，她必须定期注射病毒才能活下来，同时还要注射抗病毒血清以免变成丧尸。艾莉丝用抗病毒血清治好奥利维拉，博士将直升机等待的撤离点位置告诉艾莉丝。众人抵达集合点后却被安布雷拉部队偷袭，凯恩少校杀死博士后逼艾莉丝和追迹者战斗，声称艾莉丝的体能已被T病毒大幅强化。艾莉丝打倒追迹者，但在发现他是被安布雷拉实验变异的安德森后停手。
追迹者转为攻击凯恩少校及其手下，但为保护艾莉丝被杀。其他幸存者控制直升机后把凯恩少校推出去，后者随即被丧尸吞没，其中还有刚变成丧尸的博士。直升机飞离城市，但因核弹引爆坠毁，艾莉丝为挽救安吉拉被金属杆刺穿。电视新闻播放的录像声称浣熊市核电站堆芯熔毁导致惨剧发生，掩盖安布雷拉的所作所为。艾莉丝在安布雷拉的研究机构苏醒，然后在奥利维拉、瓦伦汀、L.J.和安吉拉的帮助下逃离。此时安布雷拉高管亚历山大·艾萨克斯博士出现，声称公司有意让艾莉丝逃走。喜欢这部电影就点我的you tube channel 台吧！以下正版的是video CD speedy video Malaysia 版.In the previous film, former security operative Alice and environmental activist Matt Addison fought to escape an underground genetic research facility called the Hive, the source of a zombie outbreak. The pair were part of an attempt to expose illegal experiments being performed there by the pharmaceutical company Umbrella Corporation. The film ended as Alice and Addison were taken into custody by Umbrella and separated.
Umbrella sends a team to the Hive to investigate what happened there; the team is overrun by zombies who quickly spread to the nearby Raccoon City. Umbrella reacts by quarantining the city and evacuating vital personnel from it. Angela Ashford, the daughter of Umbrella researcher Dr. Charles Ashford, goes missing after her security car is involved in a collision while being extracted from the city. Meanwhile, disgraced Raccoon City Police Department Special Tactics And Rescue Squad (STARS) operative Jill Valentine returns to her former precinct to encourage her fellow officers to evacuate Raccoon City. Alice awakens in a deserted hospital and wanders the city in search of supplies, while Umbrella uses the only bridge out of the area to evacuate civilians. At the bridge, Valentine meets with her former partner, Sgt. Payton Wells. A civilian turns into a zombie, biting and infecting Wells. In response to the virus reaching the bridge, Major Timothy Cain, leader of the Umbrella forces in Raccoon City, seals the exit and forces the residents to return to the city.
After being abandoned by their employer following a failed attempt to rescue a civilian, Umbrella soldiers Carlos Olivera and Nicholai Ginovaef team up with the surviving STARS operatives to repel zombie attacks. Their position is overrun, and Olivera is bitten and infected. At a separate location, Valentine, Wells, and news reporter, Terri Morales, are about to be overrun, though they are saved by Alice. Umbrella deploys a heavily mutated experimental supersoldier, Nemesis, who kills the remaining STARS members before searching for Alice. Dr. Ashford hacks into the city’s CCTV system and uses it to contact Alice and the other survivors, offering to arrange their evacuation from the city in exchange for help in locating his daughter. He makes an identical offer to Olivera and Ginovaef and explains that Umbrella intends to rid Raccoon City of the zombie infection by destroying it using a nuclear bomb.
On their way to Angela’s location, Alice and the others are ambushed by Nemesis. Valentine kills Wells after he turns into a zombie. Alice engages Nemesis but is wounded and forced to retreat separately, luring Nemesis away from the rest of the group. Valentine and Morales continue, picking up stranded civilian L.J. en route. Valentine meets Olivera, and they find and rescue Angela, although Morales and Ginovaef are both killed. Angela reveals that the zombie outbreak is the result of a virus created by her father to treat the genetic disease from which she suffers: only by regularly injecting herself with the virus is Angela able to survive, though she must also take anti-virus serum to prevent turning into a zombie. Alice uses some of the anti-virus to cure Olivera. Dr. Ashford gives Alice the location of an extraction point where a helicopter awaits. The group makes it to the rendezvous point, but are ambushed by Umbrella forces. Major Cain kills Dr. Ashford and forces Alice, whom he reveals was augmented by the T-virus, to fight Nemesis. Alice gains the upper hand over the supersoldier, though she ceases fighting after realizing that he is Matt Addison, mutated by brella’s experiments.
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